Neural Stem Cell Culture Media

  • 商品编号:Y40000          计量单位:500 ml/瓶  

  • 品牌:Cellartis

  • 商品编号:Y40001          计量单位:500 ml/瓶  

  • 品牌:Cellartis

  • 服务介绍


Neural Stem Cell Culture Media

RHB-A和RHB-Basal是两款无血清、成分完全确定的培养基,用于人类或小鼠神经干细胞(NS)的定向分化获取、维持培养、扩增、诱导分化成神经细胞等用途。RHB-A可用来培养扩增贴壁的人或小鼠的神经干细胞。在RHB-A中培养的神经干细胞保持着稳定的神经干细胞分化发育成为成熟的神经细胞的能力 (1-3) 。另外,RHB-A还用来定向分化小鼠胚胎干细胞(mouse ES)至神经前体细胞 (neural precursors) (4-6)。
RHB‐Basal不包含生长因子或是神经细胞相关的添加物。因此,可以RHB‐Basal为基础,通过添加客户认为需要的添加物来开发适合客户的特殊类型细胞的培养基。

■ 产品特点
· RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent 
   human and mouse NS cells
· RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be 
   customized by the addition of supplements 
■ 产品应用
· Derivation of mouse and human NS cells from ES cells and fetal and adult tissues
· Maintenance and propagation of adherent mouse and human NS cells
· Differentiation of mouse and human NS cells into functional neurons 
· Differentiation of mouse ES cells to neuronal precursors 
· Refer to the Data Sheet for additional examples of use
■ 产品详情
http://www.clontech.com/CN/Products/Stem_Cell_Research/Culture_and_Cryopreservation/Adherent
_Neural_Stem_Cell_Culture_Media?sitex=10022:22372:US
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP).

参考文献:
1. Ying QL, et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. 
    Nature Biotechnology
 21:183-186.
2. Conti L, et al. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell. 
    PLoS Biology
 3(9):e283.
3. Pollard SM, et al. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex 16:112-120.
4. Diogo MM, et al. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells.
    Biotechnology and Applied Biochemistry 49:105-112.
5. Pollard SM, et al. (2008) Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors 
    and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience 38:393:403.
6. Sun Y, et al. (2008) Long-term tripotent differentiation capacity of human neural stem (NS)cells in adherent culture. 
    Molecular and Cellular Neuroscience
 38:245-258.
7. Pollard SM, et al. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are 
    suitable for chemical and genetic screens. Cell Stem Cell 4:568-580.
8. Abranches E, et al. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis 
    in the embryo. PLoS ONE 4(7): e6286.
9. Fernandes et al. (2010) Hypoxia enhances proliferation of mouse embryonic stem cell-derived neural stem cells.
    Biotechnology and Bioengineering 106: 260–270.
10. Fernandes, T.G., et al. (2010) Different stages of pluripotency determine distinct patterns of proliferation, metabolism, 
      and lineage commitment of embryonic stem cells under hypoxia. Stem Cell Research 5(1):76-89.
11. Fernandes et al. (2010) Three-dimensional cell culture microarray for high-throughput studies of stem cell fate.
      Biotechnology and Bioengineering 106: 106-118.
12. Hansen, et al. (2010) Neurogenic radial glia in the outer subventricular zone of human neocortex. 
      Nature
 doi: 10.1038/nature08845.
13. Hook L, et al. (2011) Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays. 
      Neurochem Int
59(3): 432-44.


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