小鼠胚胎干细胞培养基iSTEM

商品编号:Y40010          计量单位:100ml/瓶 

  • 服务介绍

小鼠胚胎干细胞培养基iSTEM

iSTEM是一款卓越的小鼠胚胎干细胞维持培养基。该款产品包含三种小分子抑制物,从而实现阻断干细胞分化的信号通路,同时维持细胞的发育全能性(pluripotency)。 iSTEM可以被应用于难以获得ES细胞的小鼠品系,获取到ES细胞系(1)。

小鼠ES细胞在iSTEM培养基中的增殖速度与在添加了Leukemia Inhibitory Factor (LIF) 和 serum/Bone Morphogenetic Protein (BMP)的培养基中的增殖速度相似(1)。另外,在iSTEM中培养的小鼠ES细胞产生细胞聚落(clone)的能力显著提高(enhanced clonogenicity),从而可以在单细胞铺板后(single cell deposition),产生更多的干细胞克隆。

产品特点

· Completely defined formulation: serum-free, feeder-free, and cytokine-free
· Uses selective small-molecule inhibitors to block differentiation signals and maintain ES cells in a pluripotent state
· Using iSTEM medium, mouse ES cells can be efficiently maintained and propagated at ≥99% purity

产品应用

· Maintaining mouse ES cells in a pluripotent state
· Deriving mouse ES cell lines from non-permissive mouse strains

Mouse embryonic stem cells cultured in iSTEM medium.





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小鼠ES干细胞向神经细胞分化诱导培养基

Ndiff227是一款优异的用来诱导小鼠ES细胞向神经细胞分化的培养基产品,成分中包含了N2以及B-27。该产品不添加血清,成分确定(1)。

产品特点

· A complete, serum-free, ready‐to‐use medium for neural differentiation of mouse ES cells

· Supplemented with N2 and B-27

产品应用

· Neural differentiation of mouse ES cells in monoculture

· Refer to the Data Sheet for additional examples of use

Neural differentiation of mouse embryonic stem cells cultured in adherent monoculture conditions with NDiff 227 medium.

小鼠ES干细胞向神经细胞分化诱导培养基

商品编号:Y40002          计量单位:500 ml/瓶

  • 服务介绍

Neural Stem Cell Culture Media

RHB-A和RHB-Basal是两款无血清、成分完全确定的培养基,用于人类或小鼠神经干细胞(NS)的定向分化获取、维持培养、扩增、诱导分化成神经细胞等用途。RHB-A可用来培养扩增贴壁的人或小鼠的神经干细胞。在RHB-A中培养的神经干细胞保持着稳定的神经干细胞分化发育成为成熟的神经细胞的能力 (1-3) 。另外,RHB-A还用来定向分化小鼠胚胎干细胞(mouse ES)至神经前体细胞 (neural precursors) (4-6)。

RHB‐Basal不包含生长因子或是神经细胞相关的添加物。因此,可以RHB‐Basal为基础,通过添加客户认为需要的添加物来开发适合客户的特殊类型细胞的培养基。

产品特点

· RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells

· RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be customized by the addition of supplements 

产品应用

· Derivation of mouse and human NS cells from ES cells and fetal and adult tissues

· Maintenance and propagation of adherent mouse and human NS cells

· Differentiation of mouse and human NS cells into functional neurons 

· Differentiation of mouse ES cells to neuronal precursors 

· Refer to the Data Sheet for additional examples of use

Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP).

Neural Stem CellCulture Media

商品编号:Y40000          计量单位:500 ml/瓶  

商品编号:Y40001          计量单位:500 ml/瓶  


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